【摘要】 目的:以Con A诱导小鼠免疫性肝损伤模型,研究狭基线纹香茶菜对免疫肝损伤小鼠ICAM-1mRNA和蛋白表达的影响,探讨其抗免疫肝损伤的作用机理。方法:小鼠尾静脉注射ConA 25mg/kg制备免疫肝损伤模型,灌胃给予狭基线纹香茶菜水提物7d后,不同时间点取血检测血清ALT、AST水平,取肝组织,提取总RNA反转录聚合酶链式反应(RT-PCR)对肝脏ICAM-1mRNA进行半定量测定,免疫组化法检测肝组织ICAM-1蛋白的表达。结果:与正常对照组相比,模型组小鼠血清AST、ALT水平明显高于正常组,狭基线纹香茶菜水提物9.2g/kg可明显降低肝损伤小鼠血清ALT、AST水平。肝组织4h ICAM-1 mRNA和蛋白表达显著增强,至8h仍保持较高的表达。阳性药PDTC和狭基线纹香茶菜水提物大剂量组ICAM-1mRNA和蛋白表达显著低于模型组。结论:ConA损伤后,血清ALT、AST水平升高,肝组织中ICAM-1mRNA和蛋白表达明显增强,狭基线纹香茶菜组可通过降低损伤肝组织ICAM-1mRNA和蛋白的表达,而对免疫肝损伤小鼠起保护作用,这可能是其护肝降酶作用机理之一。更多还原

【Abstract】 Objective: Immune liver injury model was induced by ConA to observe influence of water-soluble extract from Isodon lophanthoides var.ge-rardianus on ICAM-1mRNA and protein expression,aiming to explore its mechanism of protective action against liver injury.Methods: Mice were challenged by a single tail intravenous injection of ConA(25mg/kg) to induce immnuce liver injury,meanwhile,water-soluble extract from Isodon lophanthoides var.gerardianus is administrated to mice for 7days,we collected blood and liver tissue at 4h and 8h time point.The level of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) in mice serum was determineated.ICAM-1mRNA and protein expression in liver tissue were detected by RT-PCR and immunohistochemistry examination.Results: Compared with normal control group,ConA can markedly increase the level of AST and ALT in serum,increase level of ICAM-1mRNA and protein expression.Extract from Isodon lophanthoides var.gerardianus at the dose of 9.2g/kg was found to significantly decrease the serum transaminase(ALT,AST),Meanwhile,the mRNA and protein expression of liver tissue were markedly lower than those of model group.Conclusion:extract from Isodon lophanthoides var.gerardianus may decrease level of ALT,AST,its Mechanism of protecting action against liver injury might be related to action of inhibiting ICAM-1 mRNA and protein expression.更多还原