【摘要】 目的检测URSA患者经主动免疫治疗前后外周血中sHLA-G的水平表达,为主动免疫治疗对URSA患者疗效评价提供一定的理论及方法学依据。方法将研究对象分为四组,URSA组、URSA主动免疫治疗组、正常早孕组及正常未孕组。用ELISA法定量检测样本中sHLA-G的表达情况,RT-PCR半定量检测外周血中sHLA-G的表达情况。结果(1)正常早孕组血清中sHLA-G的表达水平显著高于原因不明性流产组以及正常未孕组(P<0.05);URSA患者主动免疫治疗组sHLA-G的表达水平高于原因不明性流产组以及正常未孕组(P<0.05),与正常早孕组相比无统计学意义(P>0.05);(2)RT-PCR半定量检测显示URSA组及正常未孕组sHLA-G表达均低于正常早孕组及治疗组(P<0.05)。结论淋巴细胞主动免疫治疗可以提高外周血中sHLA-G的表达,主动免疫治疗能够有效治疗URSA妊娠后的流产患者。更多还原

【Abstract】 Objective: Investigating the levels of soluble human leukocyte antigen-G(sHLA-G) for unexplained recurrent spontaneous abortion(URSA) by allogenic leukocyte immunization.Intention to elucidate the possible foundation theory of allogenic leukocyte immunization.Methods: The study object were divided into four groups,including URSA,URSA of allogenic leukocyte immunization,early pregnancy period and none pregnancy.We evaluated sHLA-G levels by an enzymelinked immunosorbent assay(ELISA).RT-polymerase chain reaction(RT-PCR) for peripheral blood monouclear cells(PBMC) expressed sHLA-G in peripheral blood.Results: ①sHLA-G level in human serum by ELISA: The early pregnant group had a higher level of sHLA-G in human serums than in the URSA group and in the none pregnant women respectively(P<0.05);The level of URSA of allogenic leukocyte immunization group had a higher level of sHLA-G in human serums than in the URSA group and in the none pregnant women respectively(P<0.05),but no significantly comparing the early pregnant group(P>0.05).②sHLA-G expression in peripheral blood monouclear cells(PBMC) by RT-PCR: The early pregnant group and the URSA of allogenic leukocyte immunization group had a higher level of sHLA-G in human serums than in the URSA group and in the none pregnant women respectively(P<0.05).Conclusion: The lack of sHLA-G in female serum may lead to unexplained recurrent spontaneous abortion.The allogenic leukocyte immunization is a valid treatment for URSA.更多还原