【摘要】 目的建立柱前衍生化HPLC法测定鸦胆子油中油酸和亚油酸的含量。方法将鸦胆子以石油醚回流提取得到鸦胆子油,经柱前衍生化后,利用HPLC法测定油酸和亚油酸的含量。色谱条件:色谱柱为WatersSymmetry C1(8150 mm×3.9 mm,5μm),检测波长242 nm,流动相为甲醇-水(98∶2),柱温:28.5℃,流速:1.0 mL.min-1。十七烷酸为内标。结果油酸在0.04～0.8 mg.mL-1,亚油酸在0.02～0.6 mg.mL-1范围内具有良好的线性关系。平均加样回收率油酸为99.04%,亚油酸为96.97%。在6 min内可完成一个样品的分析。结论本法操作简便,分析时间短,为评价和控制鸦胆子油质量提供了依据。更多还原

【Abstract】 Objective To develop a pre-column derivatization HPLC method for simultaneous determination of oleic acid and linoleic acid in the oil of Fructus Bruceae.Methods After extraction and derivatization,the sample was an-lyzed on a Waters Symmetry C18(150 mm × 3.9 mm,5 μm)at 28.5 ℃,with a mobile phase of methanol-water(98 ∶ 2),at the detention wavelength of 242 nm and the flow rate being at 1.0 mL·min-1.Heptadecanoic acid was used as the internal standard.Results The linear ranges of oleic acid and linoleic acid were 0.04 ～ 0.8 mg·mL-1 and 0.02 ～ 0.6mg·mL-1,and the average recoveries of the two compounds were 99.04 % and 96.97 %,respectively.Conclusion The established method is simple and fast for the determination of oleic acid and linoleic acid in the oil of Fructus Bruceae.更多还原