【摘要】 目的:测定千斤拔属7种植物中的7种黄酮类的含量,为千斤拔类药材质量控制提供依据。方法:色谱条件为Hedera C18色谱柱(250 mm×4.6 mm,5.0μm):流速1.2 mL/min,流动相为0.5%HAc水和乙腈梯度洗脱,柱温35℃,检测波长263 nm。结果:七种黄酮类成分在90 min内达到现行分离,线性关系良好(,R2>0.9995),日内、日间精密度、稳定性和重复性的RSD≤3.0%。该法成功的应用于千斤拔药材7种黄酮的评均回收率分别为96.94%～105.29%(RSD≤2.98%)结论:本方法准确、快速、重现性好,不同品种的千斤拔药材质量存在差异,可以为研究千斤拔药材的质量标准提供依据。更多还原

【Abstract】 Objective: To develop a High-Performance Liquid Chromatography(HPLC) method for the simultaneous determination of the radix of Flemingia.Method: The separation was carried out on a Hedera ODS(4.6 mm×250 mm,5 μm) column eluted with in mobile phases of water containing 0.5% HAc and Acetonitrile gradient mode at the flow rate of 1.2 mL/min.The detective wavelength was 263nm and column temperature was 35 ℃.Results: The good separation of seven flavonoids was achieved within 90 min and showed good linear relationship(R2>0.9995),with the RSD of intra-day and inter-day presision,repeatability and stability<3.0%.The average recoveries of the seven flavonids were within 96.94%-105.29%(RSD≤2.98%).Conculsion: The developed method is simple,accurate and repeatable,and can be readily used as a powerful tool for the quality control of Flemingia.更多还原