【摘要】 目的:建立HPLC法同时测定野菊花中绿原酸和蒙花苷的含量。方法:色谱柱:Agilent Eclipse XDB-C₁₈（250 mm×4.6 mm,5μm）;流动相:甲醇-乙腈-0.05%磷酸溶液,梯度洗脱;检测波长:326 nm;流速:1.0 ml·min^-1;进样量:10μl;柱温:35℃。结果:绿原酸和蒙花苷的线性范围分别为3.476×10^-2～0.695 2μg（r=1.000 0,n=7）和8.957×10^-2～1.792μg（r=1.000 0,n=7）;提取回收率分别为98.79%（RSD=1.0%,n=6）和99.39%（RSD=0.5%,n=6）。结论:本法操作简便、快速、结果准确,可用于野菊花的质量控制。更多还原

【Abstract】 Objective:To establish a RP-HPLC method for the simultaneous determination of chlorogenic acid and buddleoside in Flos Chrysanthemi Indici.Method:An Agilent Eclipse XDB-C₁₈（250 mm×4.6 mm,5μ.m） column was used.The mobile phase was methanol-acetonitrile-0.05%H₃PO₄ with gradient elution and the detection wavelength was 326 nm.The flow rate was 1.0 ml·min^-1, the injection volume was 10μl and the column temperature was 35℃.Result:The linear response of chlorogenic acid ranged from 3.476×10^-2-0.695 2μg（r = 1.000 0,n = 7 ）,and that for buddleoside was 8.957×10_-21.792μg（r = 1.000 0,n = 7 ）.The average recovery of chlorogenic acid and buddleoside was 98.79%（RSD = 1.0%,n = 6） and 99.39%（RSD = 0.5%,n = 6）,respectively. Conclusion:The method is convenient,rapid and accurate,and can be used for the quality control of Flos Chrysanthemi Indici.更多还原