【摘要】 目的:考察流动相对高效凝胶渗透色谱法（HPGPC）测定灰树花多糖（GFP）相对分子质量（Mr）的影响因素。方法:采用HPGPC法分别以不同的流动相、盐浓度、pH测定GFP的Mr,并对测定结果进行分析。结果:分别以纯水、0.02%NaN₃、0.1mol·L^-1NaNO₃和0.1 mol·L^-1NaCl溶液为流动相进行测定,重均相对分子质量（WAMr）分别为822410、177520、29112、25066道尔顿,不同的流动相GFP的Mr有明显不同,以0.02%NaN₃较为理想;在低盐浓度(0～0.025mol·L^-1)下,Mr变化明显,在0.1～0.2mol·L^-1硫酸钠溶液中趋于平稳;在pH 3～6时,Mr随pH增加而急剧增加,在pH 6～9范围内变化不明显。结论:不同流动相、盐浓度、pH对GFP的Mr测定结果有明显影响。更多还原

【Abstract】 Objective:To investigate the influence of mobile phases on relative molecular weight（Mr）determination of Grifola Frondosa Polyaccharide（GFP）by high performance gel permeation chromatography（HPGPC）.Method:The Mr of GFP was determined by HPGPC using different mobile phases,concentrations of NaCl and pH value.Result:The Mr of GFP was 822 410,177 520, 29 112 and 25 066 Dalton determined by HPGPC using pure water,0.02%NaN₃ solution,0.1 mol·L^-1NaNO₃ solution and 0.1 mol·L^-1NaCl solution as the mobile phase,respectively.The Mr of GFP was sharply dropped using 0-0.025 mol·L^-1Na₂SO₄ as the mobile phase and then tended to be stable using 0.1-0.2 mol?L^-1Na₂SO₄.The Mr increased quickly with pH of 3 to 6,and then maintained stable with 6 to 9,while slightly increased at 10.Conclusion:Different mobile phases,concentrations of Na₂SO₄ and pH values have great influence on the Mr determination of GFP.更多还原