【摘要】 本文研究了单纯疱疹病毒(HSVⅠ)在感染与复制过程中影响宿主细胞RNA转录,蛋白表达,进而为病毒的潜伏和持续感染创造必要条件.采用基于2-DE分析,人正常肝细胞L-02细胞核不均一性核糖核蛋白H2(heterogeneous nuclear ribonucleoprotein H2,hnRNP H2)在感染HSVⅠ前后存在表达差异,构建pcDNA-hnRNP H2真核表达载体及hnRNP H2稳定转染的293T细胞系的方法,研究在核不均一性核糖核蛋白H2(hnRNP H2)稳定过表达后对HSVⅠ病毒复制周期的影响.结果表明,病毒滴度和生长曲线显示,hnRNP H2可以显著影响病毒复制周期.过表达hnRNP h2的细胞系与对照相比,其病毒滴度明显下降,hnRNP H2在HSVⅠ病毒复制中起到比较明显的抑制作用,其作用机理还有待进一步研究.更多还原

【Abstract】 Herpes simplex virusⅠ(HSVⅠ) regulating the pathway of transcription and translation modify in host cell is a very systematic and complicate system.By the analysis of 2-DE,the heterogeneous nuclear ribonucleoprotein H2 in human fetal liver cell represent distinction after the HSVⅠinfection.Establish the stable transfection hnRNP H2 cell line.Analysis post infection of different cell lines,the one-step growth curve and titer of HSVⅠ have changed dramatically.Accordingly,modulation hnRNP H2 during infections such as HSV may change the replicative cycle更多还原