【摘要】 目的:研究黄酮类成分药代动力学试验中,大鼠饲料对测定结果的干扰。方法:采用LC-ESI-MS/MS法对大鼠饲料和血浆中的黄酮进行分离,并根据其鉴定。结果:在大鼠标准饲料中检测到5个柑橘类黄酮(柚皮苷、柚皮素、橙皮苷、橙皮素和槲皮素)和2个大豆异黄酮(大豆素、大豆苷),同时在采用该饲料喂养的大鼠血浆中也检测到这7个黄酮。结论:在进行黄酮类成分的药代动力学试验时,饲料来源的黄酮会干扰血浆中该物质的测定,影响研究结果的准确性。更多还原

【Abstract】 Objective:To investigate the interference of rat feed to pharmacokinetic of flavonoids.Methods:Flavonoids in rat feed and plasma samples were separated by rapid resolution reversed-phase HPLC and characterized by LC-ESI-MS/MS analysis with an electrospray ionization source(ESI) and a triple quadrupole analyzer by fragmentation pattern.Results:Five Citrus flavonoids(naringin,naringenin,quercetin,hesperidin and hesperetin) and two soybean isoflavonoids(daizin,daidzein) were identified in common formula rat feed.The presence of these flavonoids in plasma from rats receiving the feed was also confirmed.Conclusion:The results showed the flavonoids in animal food might interfere with pharmacological and pharmacokinetic study of foreign natural compounds.This paper could be taken as a reference to pharmacological and pharmacokinetic studies of all the related substances.更多还原