【摘要】 目的:研究可用于盐酸小檗碱萃取的双水相体系。方法:先用3种双水相体系进行萃取试验,然后选择较好的一种体系进行单因素试验和正交试验。结果:向10 mL 95%乙醇和10 mL 2.2 mol/L硫酸铵溶液混合形成的双水相体系中加入质量分数为53.22%的盐酸小檗碱粗品600 mg,调节体系pH值至4,70℃水浴处理30 min,萃取率可达99.29%;将萃取液集中在一起,40℃下烘干,所得盐酸小檗碱的质量分数为88.43%。结论:该体系是适合于盐酸小檗碱萃取的双水相体系。更多还原

【Abstract】 Objective: To study the aqueous two-phase system which could be used for extraction of berberine hydrochloride.Methods: Three aqueous two-phase systems were used for extraction experiment firstly,the best one was chosen from that,and then the single factor experiment and orthogonal experiment were carried out.Results: 10 mL 95 % ethanol with 10 mL 2.2 mol/L ammonium sulfate could make a aqueous two-phase system,added 600 mg berberine hydrochloride whose purity was 53.22 % into it,regulated its pH to 4,then the system was put into water-bath heater(70 ℃) for 30 min,the extraction rate could reach 99.29 %;Collected the extraction liquid,dried it under 40 ℃,the purity of berberine hydrochloride was 88.43 %.Conclusion: This system is a suitable aqueous two-phase system for extraction of berberine hydrochloride.更多还原