【摘要】 目的:分析中药赤芍正品及混伪品ITS序列的种内相似度及特定位点序列碱基差异,为建立中药赤芍的分子鉴别标准提供思路。方法:采用PCR扩增产物直接测序的方法对市场上购得的未知来源赤芍样品S1、S2的ITS序列(包括ITS1,ITS2,5.8S)进行测定,测定结果进行BLAST比对、相似度分析、序列特异性碱基位点差异比较;用DNAMAN对GenBank上已注册的正品赤芍和混伪品赤芍的ITS序列进行分析。结果:建立了赤芍药材分子鉴别的3个指标,即根据BLAST结果定属,种内相似度缩小属内范围并定种,再通过特异性碱基位点差异最终确定并证实样品的物种;确定了样品S1来源于芍药属植物芍药Paeonia lactiflora Pall.,样品S2来源于芍药属植物川赤芍Paeonia veitchii Lynch.。结论:本文为赤芍的分子鉴别提供较为有效的思路与方法,同时为基于rDNA-ITS相似度及序列分析进行其他中药品种鉴别提供依据。更多还原

【Abstract】 Objective: To establish molecular authentication standards of Radix Raeoniae.Methods: PCR method was used to determine rDNA-ITS sequences of sample S1 and sample S2 including ITS1,5.8S,and ITS2.The sequences were analyzed by BLAST and DNAMAN software.rDNA-ITS sequences of Paeonia lactiflora Pall.,Paeonia veitchii Lynch.and their counterfeit were analyzed by DNAMAN software.Results: Established three molecular authentication standards on Radix Paeoniae:(1)The genera of samples was determined by the results of BLAST;(2)The species of samples were determined by the similarities;(3)The specific base positions confirmed the species of samples.Based on the sequence alignment and base differences,sample S1 was identified as Paeonia lactiflora Pall.,and sample S2 was identified as Paeonia veitchii Lynch..Conclusion: This paper can give an effective way of Radix Paeoniae identification.What′s more,it gives a good example for other Chinese Medicinal Materia authentication.更多还原