【摘要】 目的:采用高效液相色谱法建立了广东徐闻高良姜药材的指纹图谱。方法:采用液相色谱-质谱联用(LC-MS)进行峰识别和分离度判断,获得优化的色谱分离条件:Shim-pack VP-ODS柱(2.0 mm×250 mm,5μm),流动相A为0.1%HAC水溶液,流动相B为15%乙腈+40%甲醇+45%四氢呋喃,程序梯度洗脱;流速0.2 mL/min,柱温35℃,检测波长280 nm。结果:对所采集的10批高良姜药材进行了测定,建立了高良姜的高效液相色谱指纹图谱,确定了10个共有峰及其相对保留时间和相对峰面积。结论:本分析方法稳定、可靠,可用于高良姜药材质量的控制和鉴别。更多还原

【Abstract】 Objective: To establish the chromatography fingerprint of Alpinia officinarum by HPLC.Methods: An optimum HPLC conditions which were obtained under the assessment of LC-MS were as follows: Shim-pack VP-ODS column(2.0 mm×250 mm,5μm),0.1%HAc aqueous solution as phase A,15%Acetonitrile∶40%Methanol∶45%Tetrafuran as phase B, the flow rate was 0.20 mL/min,column temperature was 35℃ and UV detector was set at 280 nm.Results: The HPLC fingerprint of Alpinia officinarum was established,the consensus 10 peaks and their relative retention times along with the ranges of relative area were determined.Conclusion: The method is reliable and stable and can be used for the quality control and identification of Alpinia officinarum.更多还原