【摘要】 目的:研究壁虎醇提物(Gecko alcohol extract,GAE)对人食管癌EC-109细胞的增殖抑制作用及其机制。方法:MTT法检测GAE对细胞增殖抑制作用;Hoechest 33342荧光染色观察细胞形态学的改变;流式细胞术检测凋亡率;SP免疫组化法检测Fas和Caspase-3蛋白的表达。结果:GAE具有抑制食管癌EC-109细胞增殖的作用;3.0mg/mL、4.0 mg/mL GAE作用细胞48 h后凋亡率分别为20.60%和37.93%;与正常对照组相比GAE组Fas蛋白和Caspase-3蛋白的表达显著增加(P<0.05)。结论:GAE能够抑制EC-109细胞增殖并诱导其凋亡,其作用机制可能与增加Fas蛋白和Caspase3蛋白的表达有关。更多还原

【Abstract】 Objective:To explore the proliferation inhibition effects of Gecko alcohol extract(GAE) on human esophageal squamous carcinoma cell line EC-109 and its mechanism.Methods: The inhibitory effects of GAE on proliferation of EC-109 cells were measured by MTT.Nucleolus change of apoptotic cells was observed by Hoechest33342 fluorescence staining.Apoptosis rate of EC-109 cells was detected by flow cytometry.The expressions of apoptosis protein Caspase-3 and FAS in EC-109 cells were investigated by immunohistochemistry.Results: GAE had the inhibition effects on the proliferation of esophageal carcinoma cell EC-109.The apoptosis rate of EC-109 cell treated with GAE(3.0 mg/mL,4.0 mg/mL)for 48h was 20.63% and 39.73%,respectively.Compared with control group,the expression of Fas and Caspase-3 was significantly up-regulated in GAE treated group. Conclusion: GAE can inhibit the proliferation of esophageal carcinoma EC-109 cells and induce them apoptosis which may be correlated with increasing expression of protein Fas and Caspase-3.更多还原