【摘要】 目的:研究苦参素空间稳定脂质体的制备方法并考察其药剂学性质。方法:采用正交设计筛选处方,乙醇注入法制备苦参素空间稳定脂质体;用葡聚糖凝胶G-50柱分离空间稳定脂质体和游离药物,用HPLC法测定包封率;用透射电镜观察空间稳定脂质体的外观形态,并用粒径分析仪测定空间稳定脂质体的粒径和Zeta电位;进一步考察空间稳定脂质体的释放规律。结果:所得空间稳定脂质体的包封率为(85.39±1.21)%;形态为粒径均匀的球形和类球形,粒径为(156±10)nm,Zeta电位为(-39.0±3.06)mV;空间稳定脂质体的体外释放符合Higuchi方程;具有较好的稳定性。结论:优选得到的空间稳定脂质体处方和制备工艺合理、稳定,其体外释放具有缓释特点。更多还原

【Abstract】 Objective:To prepare and characterize marine sterically stabilized liposomes(Marine-SSL).Methods:Liposomes were prepared by ethanol injection technique.An orthogonal test was utilized to optimize the formulation and preparation of Marine-SSL.The unencapsulated marine and liposomes were separated by sephadex gel G-50,the encapsulation efficiency was detected by HPLC.The morphological examination of Marine-SSL was performed using transmission electron microscopy.The particle size and Zeta potential of the liposomes were measured.The in vitro release rate of marine from liposomes was tested.Results:The liposomes with spherical or ellipsoidal shape and better stability featured the encapsulation efficiency of(85.39±1.21)%,the mean partical size of(156±10)nm,and Zeta potential of(-39.0±3.06)mv.The release kinetics in vitro obeyed Higuchi equation.The stability of Marine-SSL was better.Conclusion:The selected formulation and preparation technic of Marine-SSL are rational and stable and liposomes feature a sustained release in vitro.更多还原