【摘要】 目的:对不同产地秦艽的质量和遗传多样性进行研究。方法:使用性状、显微鉴别方法进行鉴别研究,采用高效液相色谱法进行龙胆苦苷含量测定,利用RAPD技术对其遗传多样性和遗传结构进行研究。结果:表明不同产地秦艽的性状、显微特征具有差异,其有效成分龙胆苦苷的含量也具有一定的差异,各种群的遗传多样性水平依次为湟中种群(K)>环县种群(J)>子午岭种群(H)。结论:对不同地区秦艽药材的外部形态、内部构造、有效成分含量和遗传水平的顺序,为保护野生品种、确保引种栽培秦艽药材的质量提供了科学依据。更多还原

【Abstract】 Objective:To study the quality and genetic diversity in Gentiana macrophylla of different habitats for controlling the quality of Gentiana macrophylla.Methods: Main characteristics and microscopic identification were adapted to identify Gentiana macrophylla;HPLC method was applied for analysising the contents of Gentiana macrophylla.RAPD method was applied to study genetic structure and genetic diversity of Gentiana macrophylla.Results:The experiment showed that the Gentiana macrophylla of different habitats have different main charactericstics,microscopic identifications and contents,and effective ingredient contents of gentiopicroside.The levels of population genetic diversity of various groups followed by HUANGZHONG County population(K)>HUAN County population(J)>ZiWU Mountain population(H).Conclusion:The study of external morphology,internal structure,active ingredient content and genetic level of Gentiana macrophylla from different areas provides a scientific basis for protecting wild species and ensuring the quality in introducing and cultivating Gentiana macrophylla.更多还原