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老鹳草提取物对小鼠免疫性肝损伤的保护作用

Protective effect of the extracts from Herba geranium on immunological liver injury in mice

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【作者】 丁云录柳怀玉李庆杰陈声武

【Author】 DING Yun-lu1,LIU Huai-yu2,LI Qing-jie1,CHEN Sheng-wu1(1 Jilin Natural Pharmatech Co.,Ltd.,Changchun 130012,China;2 Jilin University Laboratory Animal Center,Changchun 130021,China)

【机构】 吉林天药科技有限公司国家中医药管理局三级中药药理实验室吉林大学实验动物中心

【摘要】 目的:考察老鹳草提取物对免疫性肝损伤小鼠的保护作用。方法:分别采用卡介苗(BCG)+脂多糖(LPS)、刀豆蛋白A(ConA)诱导建立小鼠免疫性肝损伤模型,灌胃给予老鹳草提取物(100,200,400mg.kg-1),观察小鼠血清天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)和乳酸脱氢酶(LDH)的活性及血清总蛋白(TP)、白蛋白(ALB)、细胞因子γ干扰素(IFN-γ)和白细胞介素4(IL-4)含量变化;检测肝组织匀浆中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;进行肝脏病理组织检查。结果:不同剂量老鹳草提取物均可明显降低小鼠血清中AST,ALT活性及肝组织匀浆MDA含量(P<0.05或0.01),并能使降低的SOD活性升高;中、高剂量老鹳草提取物(200,400 mg.kg-1)可不同程度降低血清中IFN-γ和IL-4含量及LDH活性(P<0.05)。不同剂量老鹳草提取物可使血清蛋白含量维持正常比例,肝组织损伤均有不同程度减轻。结论:老鹳草提取物对小鼠免疫性肝损伤具有一定的保护作用。

【Abstract】 Objective:To investigate the protective effect of the extracts from Herba geranium on immunological liver injury in mice.Methods:The immunological liver injury in mice was induced by BCG+LPS and ConA,respectively.The serum levels of AST,ALT and LDH and the concentrations of TP,ALB,IFN-γ and IL-4 were measured.The liver was isolated for pathohistological examination;SOD activity and MDA content in liver homogenates were determined.Results:Herba geranium extracts(100,200 and 400mg·kg-1,ig) significantly decreased the serum levels of ALT and AST,and the contents of MDA in liver homogenates(P<0.01 or 0.05);and increased the activity of SOD in liver tissue.Herba geranium extracts(200 and 400mg·kg-1,ig) decreased the serum concentrations of IFN-γ and IL-4,and the serum level of LDH(P<0.05).The concentration of total protein in serum was continuously in a normal state.Pathohistological examination showed that Herba geranium extracts effectively decreased degeneration and necrosis of liver cells.Conclusion:The extracts from Herba geranium have a protective effect on immunological liver injury in mice.

  • 【文献出处】 中国新药杂志 ,Chinese Journal of New Drugs , 编辑部邮箱 ,2011年16期
  • 【分类号】R285.5
  • 【被引频次】4
  • 【下载频次】228
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