【摘要】 目的将胶体金和肺癌靶向性多肽ZS-1制成金探针对肺癌早期血清进行筛查,通过显色差异达到早期诊断肺癌的目的,并为后期胶体金试纸的研制奠定基础。方法首先制备胶体金,将胶体金和实验室前期利用噬菌体展示肽库筛选得到的肺癌靶向性多肽ZS-1做成金探针,利用间接法检测早期肺癌患者血清中的特异性抗原。方法成功制备金探针,对肺癌早期病人血清进行筛查,以正常人血清为对照,结果肺癌血清和正常血清的显色有明显差异。结论制得的金探针可以作为肺癌早期诊断的工具,价廉、检测简便,对肺癌的早期诊断具有重要的意义。更多还原

【Abstract】 【Objective】 To screen serum of lung cancer using gold probe prepared by colloidal gold and polypeptide(ZS-1) targeted lung cancer;The color difference was observed for the early diagnosis of lung cancer.This will lay the foundation for the development for the colloid gold test paper.【Methods】 Colloidal gold was prepared,and the gold probe was prepared by colloid gold and the ZS-1 derived for Phage-displayed peptide library of our laboratory.The indirect method was used to detect specific antigen of lung cancer.【Results】 The gold probe was prepared successfully.The result of the detection of early lung cancer showed that the color had significant difference between lung cancer serum and normal serum.【Conclusion】 The prepared gold probe could be used as a tool for the early diagnosis of lung cancer,there will be important significance for the early diagnosis of lung cancer.更多还原