【摘要】 目的分析携带RHD1227A等位基因的Rh血型弱D型个体的红细胞D抗原表位。方法采用常规血清学方法检测Rh血型D、C、c、E和e抗原表型,间接抗人球蛋白试验(IAT)确认D抗原,序列特异性引物-聚合酶链反应(PCR-SSP)测定RHD基因,并检测RHD杂合型。对确认的弱D型,使用12种抗-D抗原不同表位的单克隆抗体,分析红细胞D抗原表位。结果血清学筛选和PCR-SSP检测鉴定出3例携带RHD1227A等位基因的弱D型样本,Rh小因子均为C+c+E-e+,RHD合子型鉴定均为RHD+/RHD-杂合型,提示3例个体基因型为CDe/cde。3例个体红细胞D抗原表位分析显示均具有基本完整D抗原表位,与Rh(D)阳性对照样本相同。结论发现3例RHD1227A型弱D型;不管RHD1227A等位基因表达DEL型或弱D型,其D抗原表位近似,均表达基本完整D抗原,提示该等位基因不同量的表达可能与其它调节因素有关。更多还原

【Abstract】 【Objective】 To analyze the D antigen epitopes of the weak D phenotype individuals with RHD 1227A alleles.【Methods】 Rh blood group D,C,c,E and e antigen phenotypes were tested by the routine serological methods,and the D antigen were further determioned through the indirect antiglobulin test(IAT).A sequence-specific primer PCR(PCR-SSP) method was applied for detection of RHD,and the RHD zygosity was also detected through a PCR method.To the identified samples,D antigen epitope mapping was performed using 12 monoclonal antibodies specific to different D-epitopes,respectively.【Results】 Three samples were determined as weak D phenotype,carrying RHD1227A alleles,by serological tests and the PCR-SSP detections.All samples were tested D+C+c+E-e+.The zygosity test showed all were RHD+/RHD-heterozygotes,which shows 3 donors being CDe/cde genotypes.The Red cell D antigen epitoping tests showed the sample possessing grossly intact D antigen molecules,same as the Rh(D)-positive controls.【Conclusion】 Three weak D individuals with RHD1212C>A were found.All individuals possess grossly intact D antigen in spite of RHD1212C>A expressing DEL or weak D type,which showed that the different expressions of the allele might be affected by other regulating factors.更多还原