【摘要】 目的探讨二烯丙基三硫(DATS)对急性肺损伤小鼠血清IL-18,IL-10的影响。方法 90只健康昆明小鼠,随机分为5组:①急性肺损伤组:腹腔注射大肠杆菌脂多糖(LPS);②预防组:腹腔注射DATS 7d后注射LPS;③治疗组:腹腔注射LPS后30min注射DATS;④DATS组:腹腔注射DATS 7d;⑤对照组:腹腔注射等量生理盐水。光镜观察12h点肺组织形态学改变。测定血清中IL-18及IL-10在2h和6h含量。结果 DATS预防组肺泡间渗出及炎性细胞浸润较ALI组明显减轻。其血清中IL-18含量均明显低于ALI组(P<0.01),IL-10含量均明显高于ALI组(P<0.01)。结论预防性应用DATS可减轻LPS诱导的ALI小鼠肺组织炎症反应。并能抑制LPS诱导的小鼠血清中炎性细胞因子IL-18的生成,而促进抗炎性细胞因子IL-10的生成。更多还原

【Abstract】 【Objectives】 To observe the effect of DATS on the inflammatory cytokines IL-18,IL-10 in serum of LPS-induced ALI mice.【Methods】 Ninety healthy Kun-ming mice were randomly divided into five groups: ALI group: mice were infused LPS intraperitoneally;DATS prevention group: mice were infused DATS intraperitoneally for 7 days and then received LPS injection;DATS treatment group: mice were infused LPS intraperitoneally and then received DATS injection 30 min later;DATS group: mice received DATS injection intraperitoneally for 7 days and without LPS injection;Control group: mice were received the same bulk of sodium chloride injection.The histopathology changes of lung tissues at 12 h were examined under light microscopy.The levels of IL-18 and IL-10 in the serum were detected with ELISA at 2 h and 6 h.【Results】 In DATS prevention group,the concentration of IL-18 in the serum decreased significantly while the concentration of IL-10 increased significantly at 2 h and 6 h(P <0.01 vs ALI group).【Conclusions】 Pretreatment with DATS could alleviate the degree of pulmonary inflammation in LPS-induced ALI mice.Pretreatment with DATS could inhibit the production of IL-18 and increase the production of IL-10 in the serum in LPS-induced ALI mice.更多还原