【摘要】 选择原产中国的98份亚洲栽培稻和125份普通野生稻为材料,对叶绿体中atpA序列、rps16内含子序列、trnP-rp l33间隔区、trnG-trnfM序列、trnT-trnL间隔区序列的5段高突变序列进行测序,利用生物信息学方法进行比对分析,绘制Network网络图,构建系统发育树。结果表明,普通野生稻的Indel和SNP数目均比亚洲栽培稻多,序列多样性丰富;基于单倍型的Network网络图和系统发育树可将所有参试材料归为3个类群,类群I主要为粳稻与普通野生稻,类群II主要为籼稻,类群III主要为普通野生稻,而类群II和类群III亲缘关系较近,提示粳、籼两个亚种可能由偏粳、偏籼的普通野生稻分别进化而来,支持二次起源学说;所有与亚洲栽培稻亲缘关系较近的普通野生稻均来源于华南地区,支持华南地区为我国亚洲栽培稻起源中心的论点。更多还原

【Abstract】 To study the origin and evolution of Asian cultivated rice(Oryza sativa L.),98 accessions of Asian cultivated rice and 125 accessions of common wild rice(Oryza rufipogon) were selected for sequencing analysis.Through sequencing the atpA gene,rps16 intron,trnP-rpl33 inter spacer,trnG-trnfM inter spacer and trnT-trnL inter spacer in chloroplast genome in rice,the haplotype network and phylogenetic tree were made with bio-information software.The results showed that common wild rice had more Indels and SNPs compared with the Asian cultivated rice.Both the haplotype network and phylogenetic tree divided all test materials into three groups.GroupⅠconsisted by accessions from the Japonica subspecies and the common wild rice,Group Ⅱ included mainly accessions of Indica subspecies,while Group Ⅲ were as the accessions of the common wild rice.Importantly,Group Ⅱ and Group Ⅲ had a close relationship,which indicated that the Japonica and Indica Subspecies were originated from Japonica-like and Indica-like common wild rice separately,supporting the Double Domestication Hypotheses of Asia cultivated rice.All the materials of common wild rice with a close relationship to Asian cultivated rice were identified to be collected from South China,implicating South China might be the origin center of Asian cultivated rice.更多还原