【摘要】 L-古洛糖酸-1,4-内酯氧化酶(L-gulono-1,4-lactone oxidase,GLOase)是维生素C合成途径中最后一步关键酶,小鼠(Musmusculus)编码GLOase的gulo基因转化拟南芥(Arabidopsis thaliana)的转基因株系中维生素C含量最高为5.74μmol·g-1(FW),是野生型的3.46倍、转p2301空载体对照的3.19倍。30%聚乙二醇(PEG-6000)模拟干旱胁迫的不同时间梯度中,幼苗期转基因拟南芥丙二醛含量低于同样处理下野生型和对照组拟南芥。不同NaCl浓度的盐胁迫下,转基因拟南芥在子叶期比野生型、对照组平均根长更长、侧根发育更好;幼苗期莲座叶长势更好、丙二醛含量更低。结果显示过量表达GLOase的转基因拟南芥在维生素C含量提高的同时,抗胁迫能力有所增强。更多还原

【Abstract】 L-gulono-1,4-lactone oxidase(GLOase) was the last key enzyme in native vitamin C synthesis.Gulo which encoded GLOase in mice(Mus musculus) was transformed into Arabidopsis thaliana.Transgenic A.thaliana overexpressing GLOase accumulated vitamin C which was 3.46-fold of WT and 3.19-fold of p2301 blank transformed control.During 30% PEG-6000 mocked drought stress,lower malondialdehyde(MDA) content was witnessed in young transgenic A.thaliana plants,which was a demonstration of better anti-drought ability.NaCl salt stress assay showed the enhanced salt tolerance of transgenic A.thaliana plants concerning root development and MDA level.The results indicated that overexpressing GLOase increased vitamin C level and stress tolerance of transgenic A.thaliana plants.更多还原