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蜡样芽胞杆菌及毒素引起食物中毒的实验室检测

Laboratory testing of a case of food poisoning caused by bacillus cereus infection

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【作者】 陈慧燕马雪莲

【Author】 CHEN Hui-yan,Ma Xue-lian(Wenzhou Center for Disease Control and Prevention,Wenzhou 325027,China)

【机构】 浙江省温州市疾病预防控制中心

【摘要】 目的:探讨食物中毒实验室检测机制。方法:采集中毒者肛拭子、剩余食物,带回实验室进行金黄色葡萄球菌、蜡样芽胞杆菌检验。结果:4份剩余食物其中3份样品检出蜡样芽胞杆菌,并蜡样芽胞杆菌菌落总数>105 cfu/g,所有样品的细菌生化反应结果一致。并在剩余食物中检出蜡样芽胞杆菌肠毒素。肛拭子和剩余食物均未检出金黄色葡萄球菌。结论:按WS/T.82-1996诊断标准,可判定此次是由蜡样芽胞杆菌所引起的食物中毒。

【Abstract】 Objective:Explore the mechanism of laboratory testing of food poisoning.Methods:Pathogensof Bacillus Cereus and Staphylococcus from patient’s foods and anal swat were collected for detection.Rusults:In the 4 samples of leftover,Bacillus Cereus were found in 3 samples with aerobic plate count of Bacillus Cereus >105 cfu/g,chemical reaction of these samples were same.All these isolates have Bacillus Cereus enterotoxin.Staphylococcus was not detected from all of these.Conclusion:The Bacillus Cereus and Bacillus Cereus enterotoxin could cause the food poisoning event according to "WS/T.82-1996".

  • 【文献出处】 中国卫生检验杂志 ,Chinese Journal of Health Laboratory Technology , 编辑部邮箱 ,2011年08期
  • 【分类号】R155.31
  • 【被引频次】12
  • 【下载频次】312
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