【摘要】 目的:了解不同来源的金黄色葡萄球菌(Staphylococcus aureus,SA)分离株产肠毒素的情况,以及主要肠毒素SEA、SEB、SEC、SED、SEE基因携带状况。方法:采用小型VIDAS全自动荧光酶标免疫法对46株分别从医院消毒监测样品、公共场所监测样品、食品监测样品以及食物中毒病人粪便和呕吐物中分离到的SA菌株进行肠毒素检测,再采用PCR技术对产肠毒素的菌株进行基因分型。结果:46株SA菌株中,携带肠毒素的为22株,检出率为47.83%。其中以SEA、SEB检出率较高,分别为31.82%和27.27%。不同来源SA分离株携带肠毒素基因的比例不同,24株来自医院消毒监测样品分离株中检出率为41.67%(10/24);4株来自公共场所监测样品分离株中检出率为50.00%(2/4);12株来自食品监测样品分离株中检出率为33.33%(4/12);6株食物中毒样本分离株中检出率为100%(6/6)。结论:46株SA分离株中产肠毒素为22株,比例较高,而且携带肠毒素基因的类型较多。试验表明,VIDAS与PCR技术应用于检测SA分离株中肠毒素基因简单、快速,适合基层疾控部门应用。更多还原

【Abstract】 Objective:To detect enterotoxin genes(SEA、SEB、SEC、SED、SEE) in Staphylococcus aureus isolates from different sources and characterize genotype that of isolates.Methods: 46 S.aureus isolates were isolated from hospital disinfection/public/food monitoring sample and the vomit and excreta from food poisoning patient,then the enterotoxin genes were detected by VIDAS and characterized genotype by PCR.Results: The positive rate of the enterotoxin gene in isolates was 47.83%,and the most frequently found genes were SEA(31.82%) and SEB(27.27%).Enterotoxin gene from different sources had different detection rates.The enterotoxin genes were positive in 10 of 24 strain from hospital disinfection monitoring samples,2 of 4 strain from public monitoring sample,4 of 12 strain from food monitoring sample and 6 of 6 strain from the vomit and excreta from food poisoning patient,then the positive rate were 41.76%,50.00%,33.33%,and 100%,respectively.Conclusion: 22 of 46 strains can be detected kinds of S.aureus enterotoxin genes,then the positive rate was high.VIDAS and PCR can be applied to detect enterotoxin genes simply and rapidly,then the methods could be used in grass root CDC conveniently.更多还原