【摘要】 目的:扩增2009年镇江市肠道病毒71型(EV71)vp1基因的核苷酸序列,同相关毒株序列进行同源性比对和进化分析,以了解2009年镇江市肠道病毒71型的基因组序列特征及可能的传播来源。方法:采集临床手足口病(HFMD)患者咽拭子标本,抽提病毒RNA,通过巢式PCR扩增肠道病毒71型vp1基因的核苷酸序列。利用Clustal X和MEGA5.0分析软件,进行同源性分析和构建系统发生树。结果:序列进化分析表明,测序获得的11株病毒基因与C基因型代表株比较接近,尤其与C4基因亚型最为相近,并且可进一步划分为C4a进化枝。说明本文的11株病毒皆属于EV71病毒C基因型、C4a亚型。结论:2009年镇江市的EV71病毒株可能与阜阳分离的EV71病毒有相同的起源,均属于C4a亚型,并且C4a亚型的EV71病毒在中国大陆有较广泛的分布和传播。更多还原

【Abstract】 Objective:To phylogenetically analyze enterovirus 71(EV71) strains isolated from Zhenjiang in 2009 by amplifying vp1 gene.Methods:The throat specimens were selected from different patients of HFMD,and 11 nucleotide sequences of EV71 vp1 regions were successfully determined by RT-nested-PCR and sequencing.EV71 genotypes were analyzed using bioinformatic programs.Results:The sequence analysis showed that the 11 strains belong to C genotype,C4a subgenotype.Conclusion:The strains isolated from Zhenjiang are closely related to those isolated from Fuyang.All of them belong to C4a subgenotype and C4a subgenotype widely distributes and spreads in mainland.So it is important to enhance EV71 gene analysis and search after evolution track for EV71 in China.更多还原