【摘要】 在广东发现了可能被番茄斑萎病毒属(Tospovirus)病毒侵染的西瓜,采用ELISA和RT-PCR法对该西瓜病样进行了检测,西瓜病叶粗汁液不与番茄斑萎病毒(Tomato spotted wilt virus,TSWV)和西瓜银斑驳病毒(Watermelon silver mottle virus,WSMoV)的血清发生反应;利用引物J13/UHP通过RT-PCR可以扩增出约1400 bp的基因片段,该片段包括一个840 bp的核衣壳蛋白ORF,其推导的氨基酸序列与已报道的Melon yellow spot virus(MYSV)NP基因氨基酸序列的同源率都为99%,进化树分析表明侵染广东西瓜的病毒(命名为MYSV-GZ)属于Tospovirus的MYSV血清组。更多还原

【Abstract】 A watermelon sample possibly infected by tospovirus in Guangdong was detected by ELISA and RT-PCR.Crude extracts from yellow spot watermelon leaves were not react with antisera of Tomato spotted wilt virus(TSWV) and Watermelon silver mottle virus(WSMoV) by ELISA.When they were tested by RT-PCR using primer pair J13/UHP,about 1400bp fragment was amplified,which contained 840bp ORF of nucleocapsid protein(NP).Sequence analysis showed that the NP shared 99% identity at the amino acid level with those reported NP of Melon yellow spot virus(MYSV),phylogenetic analysis confirmed that the virus infected watermelon in Guangdong(designated as MYSV-GZ) was belonged to MYSV serogroup of Tospovirus.更多还原