【摘要】 目的研究阿托伐他汀及辅酶Q10通过抗氧化作用改善心衰大鼠的作用机制。方法结扎大鼠左冠状动脉前降支并饲养6周的24只存活大鼠随机分为假手术组(Shamgroup,n=6)、模型组(Model group,n=6)、阿托伐他汀组(atorvastatin group,n=6)、阿托伐他汀+辅酶Q10组(atorvastatin+coenzyme Q10 group,n=6),连续灌胃给药5周后测定大鼠血流动力学参数,全心及左室肥厚指数(HW/BW,LVHW/BW),血清SOD活性及MDA含量,RT-PCR法测定非梗死区心肌组织UCP2 mRNA表达水平。结果联合应用阿托伐他汀及辅酶Q10更能明显升高左心室内压最大上升、最大下降速率(±dp/dtmax)及SOD活性,降低左心室收缩压(LVSP)、左心室舒张末压(LVEDP)、心肌肥厚指数、血清MDA的含量及下调UCP2 mRNA表达水平(P<0.05-0.001),但对心率(HR)、收缩压(SBP)、舒张压(DBP)无明显影响(P>0.05)。结论阿托伐他汀联合辅酶Q10可能通过抗氧化作用,下调心肌梗死后心衰大鼠心肌细胞内UCP2 mRNA的表达水平改善心衰心室重构过程中能量代谢。更多还原

【Abstract】 Objective To investigate the antioxidation effects of atorvastatin and coenzyme Q10 on experimental heart failure in rats after myocardial infarction(MI).Methods The rat model of heart failure was established by left anterior descending coronary artery deligation.After 6 weeks,24 surviving rats were divided into sham,model,atorvastatin group and atorvastatin+coenzyme group at random,6 rats in each,and were treated with medicines by intragastric administration respectively.At 12 weeks,we measured left ventricular end-diastolic pressure(LVEDP),left ventricular systolic pressure(LVSP),+dp/dtmax and-dp/dtmax of left ventricular pressure,the activity of serum superoxide dismutase(SOD) and the content of serum malondialdehyde(MDA) were quantified.The heart weight(HW)and left ventricular heart weight(LVHW)were weighed.HW/ BW and LVHW/BW were calculated.Furthermore,the mRNA levels of UCP2 were detected by RT-PCR in noninfarction zone.Results Atorvastatin association with coenzyme Q10 increased ±dp/dtmax(P<0.05～0.01)and the activity of serum superoxide dismutase,whereas decreased significantly LVSP and LVEDP,the content of MDA in serum,myocardial hypertrophy index,downregulated the mRNA expressiones of UCP2 in noninfarction zone(P<0.05～0.001).But no influence on HR,SBP and DBP(P>0.05).Conclusion Atorvastatin association with coenzyme Q10 has improve energy metabolism by downregulating the mRNA expressiones of UCP2 on heart failure after AMI in rats,which may be related to scavenging the oxygen free radicals formed.更多还原