【摘要】 目的探讨FTY720对体外培养MCF-7细胞增殖及凋亡的影响。方法体外培养MCF-7细胞,将其培养液中加入不同浓度的FTY720,继续培养48小时,MTT法检测MCF-7细胞的增殖情况;瑞士-姬姆萨染色观察细胞形态变化;流式细胞仪检测FTY720对MCF-7细胞的细胞周期、凋亡率的影响。结果当FTY720浓度为6 400 ng/ml时,体外培养MCF-7细胞的增殖受到明显抑制,抑制率为62.0%(P<0.05),而且抑制率呈浓度依赖性;镜下可见细胞出现凋亡的形态;流式细胞仪检测分析显示FTY720可将MCF-7细胞阻滞于G1期,并促进其发生凋亡,凋亡率呈浓度依赖性。结论在体外培养的MCF-7细胞培养液中加入一定浓度的FTY720,能明显抑制该细胞的增殖,调节该细胞周期并诱导其凋亡。更多还原

【Abstract】 Objective To study effect of FTY720 on in vitro MCF-7 proliferation and apoptosis.Methods MCF-7 cells were cultured in vitro and different concentrations of FTY720 were added to its culture medium,continued to culture for 48 hours,determine MCF-7 cell proliferation by MTT assay;observe cell modality by Switzerland-Giemsa staining;determine cell cycle and apoptosis rate by Flow cytometry Detection.Results FTY720 at concentration of 6 400 ng/ml could significantly suppress in vitro MCF-7 proliferation(P<0.05)with the inhibition rate of 62.0% and in a concentration-dependent manner,cells appeared apoptotic modality,MCF-7 cells were arrested in G1 phase and promote their apoptosis and the apoptotic rate in a concentration dependence.Conclusion Adding a certain concentration of FTY720 to In vitro MCF-7 cell culture medium significantly inhibits the cell proliferation,regulats the cell cycle and induces their apoptosis.更多还原