【摘要】 从屠宰场收集水牛卵巢上抽取的卵母细胞,经体外成熟培养后分别进行孤雌激活(PA)和体外受精(IVF),将获得的PA或IVF胚胎分别培养于添加浓度均为5%的牛血清白蛋白(BSA)、胎牛血清(FBS)、发情水牛血清(OBS)、发情牛血清(OCS)和混合血清(OCS与FBS按1:1混合)的培养液中培养7～9 d,并观察胚胎的体外发育情况。结果,PA胚胎培养在OCS和混合血清组的卵裂率(73.52%,68.07%)和囊胚率(30.59%,27.73%)均显著高于BSA(38.37%,6.40%)、OBS(44.97%,10.05%)和FBS(52.02%,17.04%)组(P<0.05);IVF胚胎的卵裂率和囊胚率以OCS组最高(62.50%,22.62%),显著高于BSA(30.50%,6.38%)、OBS(35.48%,7.10%)和FBS组(40.11%,11.76%,P<0.05),但与混合血清组(60.42%,21.35%;P>0.05)差异不显著。结果表明,在水牛胚胎体外培养体系中添加5%的OCS或混合血清均能有效地促进水牛胚胎的体外发育。更多还原

【Abstract】 This study was conducted to examine the effects of different serum on the in vitro development of buffalo embryos.Early buffalo embryos derived from parthenogenetic activation(PA) or in vitro fertilization(IVF) were cultured in the medium with 5%BSA,fetal bovine serum(FBS),oestrus serum of buffalo(OBS),oestrus serum of cattle (OCS) Or mixed serum(OCS:FBS= 1:1),respectively.And then,the developmental potential of buffalo embryos was evaluated after cultured in 30μL drop of granulosea cell monolayers for 7-9 days.The result showed that the cleavage(73.52%,68.07%vs.38.37%,44.97%,52.02%;P<0.05) and blastocyst rate(30.59%,27.73%vs 6.40%,10.05%and 17.04%) of PA embryos cultured in with OCS or mixed serum groups were significantly higher than those of embryos treated with BSA,OBS,FBS groups.Also,the highest cleavage rate and blastocyte development rate of IVF embryos were the embryos culture in OCS group(62.50%,22.62%),which significantly higher than that of embryos cultured in BSA(30.50%,6.38%),OBS(35.48%,7.10%;P<0.05) and FBS(40.11%, 11.76%) groups,but there was no significant difference in either cleavage or blastocyte development rate compared with mixed serum group(60.42%,21.35%,P>0.05).The results indicated that addition 5%OCS or mixed serum in the culture medium could improve the development of buffalo embryos effectively.更多还原