【摘要】 研究了云芝子实体氯仿提取物(CVFC)对人肝癌细胞株HepG2的体外抑制作用,并初步探讨了抑瘤机制。MTT结果显示,CVFC对HepG2细胞有明显的抑制作用,并呈明显的量效关系,半数抑制浓度为(75.83±3.96)μg.mL-1。荧光显微镜和透射电镜形态观察发现CVFC处理可使细胞发生细胞皱缩、核碎裂等凋亡特征;Annexin V/PI双染结果证实有胞膜磷脂酰丝氨酸的翻转,经150μg.mL-1 CVFC处理HepG2细胞24 h后早期凋亡率为17.82%,晚期凋亡率为22.59%,均显著高于对照组5.35%和3.75%。这些结果表明CVFC具有体外抑制HepG2细胞的活性,其抑瘤机制与诱导肿瘤细胞凋亡有关。更多还原

【Abstract】 In this report,in vitro the inhibition and cytotoxic effects of a chloroform extract from Trametes versicolor fruiting body(CVFC) was studied using a liver carcinoma HepG2 cell line.MTT assay results showed the CVFC inhibited HepG2 cells growth in a dose-dependent manner.The IC50 was found as(75.83±3.96) μg·mL-1.Cell morphological observation using fluorescent microscope and transmission electron microscope revealed that the CVFC treated cells presented typical apoptotic characteristics,including cell shrinkage,nuclear condensation and fragmentation.Annexin V/PI staining assay confirmed that when HepG2 cells treated with CVFC at a concentration of 150 μg·mL-1 for 24 h,the cell membrane phosphatidylserine flipped out,which was a golden mark of apoptosis.The percentage of early and late apoptosis cells were 17.82% and 22.59% respectively,much higher than that of the control(5.35% and 3.75% respectively).These results suggested that CVFC had an inhibition effect on HepG2 in vitro and mechanism may be through inducing apoptosis.更多还原