【摘要】 目的建立参类保健食品人参总皂苷比色测定新方法。方法采用大孔吸附树脂纯化样品,人参皂苷Re为标准品,通过蒽酮与人参皂苷分子中的糖基反应进行比色法测定。结果人参皂苷5～200μg/ml范围内呈线性关系,y=0.369+0.994x,相关系数r=0.999 5,回收率90.7%～104.2%,相对标准差3.50%～6.42%。结论该方法简便、快速、准确,能满足参类保健食品中总皂苷的测定。更多还原

【Abstract】 Objective To establish a new colorimetric assay method for the determination of total ginsenoside in Ginseng health foods.Methods Samples were purified by macroporous adsorption resin,the reaction of anthrone with glycon in ginsenosides was assayed by colorimetric method.Ginsenoside Re was used as a reference material.Results There was a good linearity in the range of 5-200 μg/ml of ginsenoside,the regression equation was y=0.369+0.994x,the correlation coefficient r=0.999 5.The recovery rate was 90.7%-104.2%.The relatively standard deviation was 3.50%-6.42%.Conclusion The method was simple,fast,accurate and precise,which could meet the requirement for the determination of total ginsenoside in health foods.更多还原