【摘要】 目的Axl和Tyro-3受体在脑内有区域性的分布,但两者在中枢神经系统中的生物学功能尚不明确。本研究旨在探讨Axl和Tyro-3受体在神经元分化中的作用。方法PC12细胞分别转染CMV-Axl-eGFP、CMV-Tyro-3-eCFP和CMV-eGFP质粒后,给予神经生长因子(nerve growth factor,NGF)诱导,观察绿色荧光蛋白和青色荧光蛋白的表达和分布。结果Axl-eGFP和Tyro-3-eCFP的表达随着NGF作用时间的延长而逐渐上调,并且荧光蛋白在细胞内的定位也发生变化。作为对照组,CMV-eGFP转染的PC12细胞并没有出现此变化。此外,过表达Axl和Tyro-3能够促进PC12细胞的突起生长。结论Axl和Tyro-3受体的表达受NGF调控,其过表达可能参与了PC12细胞的分化。更多还原

【Abstract】 Objective Tyro-3 and Axl receptors are expressed in brain in a region-specific manner and their bioactivities in the central nervous system remain still elusive.The aim of the present study was to investigate their functions in neuronal differentiation.Methods PC12 cells overexpressing Tyro-3 or Axl were established by transfection with full-length CMV-Tyro3-eCFP or CMV-Axl-eGFP plasmid,respectively.CMV-eGFP plasmid served as a control vector.After that,the fluorescence intensity and distributions of green fluorescent protein (GFP) and cyan fluorescent protein (CFP) in the cells with or without nerve growth factor (NGF) treatment were real-time monitored.Results Expressions of Tyro-3 and Axl receptors were under the regulation of NGF and associated with neuronal differentiation.This was not observed in CMV-eGFP-transfected PC12 cells.Besides,confocal microscopy revealed that NGF affected intracellular localization of full-length Axl-eGFP and Tyro-3eCFP in PC12 cells.Moreover,the development of outgrowth of differentiated PC12 cells under stimulation of NGF was promoted by overexpression of Tyro-3 or Axl.Conclusion Expressions of Tyro-3 and Axl receptors are under the regulation of NGF and are involved in NGF-induced neuronal differentiation of PC12 cells.更多还原