【摘要】 目的:采用HPLC建立广西产桑椹的指纹图谱。方法:采用Agilent Zobax C18(4.6 mm×250 mm,5μm)色谱柱;以乙腈(A)-0.1%磷酸(B)为流动相进行梯度洗脱,检测波长360 nm,流速1.0 mL.min-1,柱温30℃。结果:检测了广西不同来源10批桑椹药材,标定了10个共有峰,相似度较高,建立的桑椹指纹图谱稳定性、重复性好。结论:桑椹HPLC指纹图谱可用于桑椹药材的鉴别及质量控制。更多还原

【Abstract】 Objective: To establish the chromatography fingerprint of Morus alba L.by HPLC.Method:The samples are separated on Agilent Zobax C18(4.6 mm×250 mm,5 μm) with acetonitrile-0.1% phosphoric acid as the mobile phase,gradient eluent,at the flow rate of 1.0 mL·min-1 at 30 ℃,the UV detection is seted at 360 nm.Result: Ten samples of M.alba in different origin of Guangxi was detected and 10 peaks in the chromatogram are common,there is a high similarity,the reproducibility and stability is good.Conclusion: The HPLC fingerprint can be used for identification of M.alba as well as quality control.更多还原