【摘要】 目的:通过正交试验设计法优选甘蔗叶粗多糖的提取工艺条件。方法:采用L9(34)正交试验设计法优化甘蔗叶提取工艺,通过分光光度法,以苯酚-硫酸法测定粗多糖含量,以多糖提取量为指标优选甘蔗叶粗多糖最佳提取工艺。结果:甘蔗叶粗多糖提取的最佳条件为料液比1∶30,于100℃提取3次,每次5 h。结论:验证试验表明优选出的提取工艺稳定、合理、可行。更多还原

【Abstract】 Objective:To optimize the extraction process of polysaccharide from sugarcane leaves by orthogonal design.Method:The yield of polysaccharide as index to optimize extraction process of polysaccharide from sugarcane leaves by orthogonal test with spectrophotometry.The content of polysaccharide was determined by phenol-sulfuric acid method.Result:The optimum extraction technology was as follows:extracted 3 times at 100 ℃ with 30 times amount of deionized water for 5 h per time.Conclusion:The verification test showed that the optimum extraction process was stable,reasonable and feasible.更多还原