【摘要】 目的:建立红腺忍冬叶指纹图谱,探讨指纹图谱的建立方法和应用。方法:RP-HPLC法,采用C18 ODS2柱(4.6mm×250 mm,5μm),甲醇-乙腈-0.05%磷酸梯度洗脱,流速0.5 mL.min-1,检测波长328 nm,柱温25℃。结果:对11批红腺忍冬叶的样品图谱数据进行了数理统计,建立红腺忍冬叶的指纹图谱标准,并将其应用于其他种忍冬样品的定性鉴别和质量评价。结论:扩展了中药指纹图谱的建立方法和在定性识别、定量、半定量评价其他样品的应用。更多还原

【Abstract】 Objective:To establish the fingerprint chromatogram of leaf Lonicera hypoglauca,and research the establishment method and application to the fingerprint chromatogram.Method: RP-HPLC was used.The samples were separated on a C18ODS2 column(4.6 mm×250 mm,5 μm) and detected at 328 nm.The column temperature was set at 25 ℃.The flow rate was 0.5 mL·min-1,methanol,acetonitrile and 0.05% phosphoric acid was used as a linear gradient elution of the mobile phase.Result: The fingerprint chromatogram based on the data of 11 batches of leaf L.hypoglauca was set up and had been applied in Samples-recognition and quality assessment.Conclusion:The method and application of fingerprint chromatogram in Chinese medicine were extended to recognize and access other samples.更多还原