【摘要】 目的:优化桑椹中芦丁的提取条件并建立芦丁的HPLC测定方法。方法:采用单因素考察法优化桑椹中芦丁的提取条件,采用HPLC法测定芦丁的含量,色谱柱为Agilent Zobax C18(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸(19∶81)为流动相,流速1.0 mL.min-1,检测波长360 nm,柱温30℃。结果:芦丁最佳提取条件为料液比1∶25,50%乙醇回流60 min,芦丁在0.1～2.0μg有良好线性关系(r=0.999 9)。平均回收率为102.4%,RSD 1.8%(n=6)。结论:该方法简便、准确、可靠,可作为桑椹中芦丁含量测定的方法。更多还原

【Abstract】 Objective:Optimize the extract condition of rutin in Morus alba to establish a quantification method for rutin.Method: Single-factor analysis was applied to optimize the extract condition of rutin in Morus alba HPLC was used in the determination of rutin on C18 column(4.6 mm × 250 mm,5 μm) with a mixture of acetonitrile-0.1% phosphoric acid(19:81) as a mobile phase at the flow rate of 1.0 mL.min-1.Rutin was detected at 360 nm,column temperature was 30 ℃.Result: The optimal extract condition was the solid-to-liquid ratio 1:25,50% ethanol refluxed for 60 min.The linearity of rutin was in the range of 0.1-2.0 μg.The average recovery rate of rutin was 102.4% with RSD 1.8%(n = 6).Conclusion: The determination method was simple,accurate and reliable,it could be used as the determination method of rutin in Morus alba.更多还原