【摘要】 目的:研究巴豆生物碱(croton seed alkaloid,CA)对人肝癌SMMC-7721细胞的诱导凋亡作用并探讨其对凋亡相关蛋白Bax和Bcl-2表达的影响。方法:不同浓度CA分别处理SMMC-7721细胞24～48 h后,MTT法检测细胞的生长活性,流式细胞仪检测细胞凋亡率,Western blot检测凋亡相关蛋白Bax,Bcl-2的表达。结果:10～200 mg.L-1 CA均能抑制SMMC-7721细胞增殖(P<0.05),其抑制率与作用时间及浓度相关;CA作用SMMC-7721细胞24 h后,对照组凋亡率为(0.26±0.14)%,10,50,100,200 mg.L-1 CA组细胞凋亡率分别为(5.85±0.47)%,(17.70±1.23)%,(33.25±2.08)%,(49.52±1.85)%,与对照组比较,差异有统计学意义(P<0.05);Western blot结果提示经CA作用24 h后,随着药物浓度的增加,Bax的表达逐渐增加,而Bcl-2的表达逐渐减少。结论:CA能抑制SMMC-7721细胞的生长并促进其凋亡,其发生与Bcl-2蛋白的减少和Bax蛋白表达增多有关。更多还原

【Abstract】 Objective:To study the effect of Crotonis Fructus alkaloid(CA) on apoptosis and the expression of Bcl-2 and Bax in human hepatoma SMMC-7721 cells.Method:After treatment with CA for 24-48 h,MTT assay was used to determine the cell growth inhibitory rate in vitro,flow cytometry was used to detect the apoptosis of SMMC-7721 cells before and after CA treatment.Western blot was used to detect the expression of Bcl-2 and Bax.Result:CA inhibited the growth of SMMC-7721 cells in a dose-and time-dependent manner.After treatment with 50-200 mg·L-1 CA for 24 h,the number of apoptosis cells was increased dose-dependently from(0.26±0.14)% in the control group to(5.85±0.47)%,(17.70±1.23)%,(33.25±2.08)% and(49.52±1.85)% in CA groups,respectively.By Western blot analysis,we found the level of Bax was up-regulated,whereas,the level of Bcl-2 down-regulated.Conclusion:CA can efficiently inhibit the proliferation and induce apoptosis in SMMC-7721 cells.Its mechanism is likely related to the down-regulation of Bcl-2 and up-regulation of Bax.更多还原