【摘要】 目的:观察藤茶提取物(TF)抗慢性肝纤维化的作用及机制。方法:将Wistar大鼠随机分为TF高(200 mg.kg-1)、中(100 mg.kg-1)、低(50 mg.kg-1)剂量组,秋水仙碱(colchicine,Colc,0.1 mg.kg-1)组,模型组和正常组,ig给药。给药同时各组皮下注射(sc)四氯化碳(CCl4)造模,每周2次。于10周后,检测大鼠血清丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST),碱性磷酸酶(AKP),白蛋白(ALB),球蛋白(GLB),肝组织羟脯氨酸(Hyp),丙二醛(MDA),谷胱甘肽过氧化物酶(GSH-Px)及苏木精-伊红(HE)染色后的肝组织病理变化。结果:与模型组比较,TF高、中、低剂量组均能明显降低肝纤维化大鼠血清中ALT,AST,AKP,GLB的含量(P<0.01)及肝组织中MDA和Hyp的含量(P<0.05),明显提高血清ALB水平(P<0.01)及肝组织GSH-Px活性(P<0.01);肝脏组织学检查表明,TF可明显改善肝组织病理损伤程度,其作用呈剂量依赖性。结论:TF对实验性慢性肝损伤具有保护肝细胞,减少肝损伤,抗肝纤维化作用。更多还原

【Abstract】 Objective:To investigate the effects of Tengcha extract(TF) on hepatofibrosis induced by chronic hepatic injury.Method: Wistar rats were randomized into six groups: TF treatment(200,100 and 50 mg.kg-1) groups,colchicines treatment(Colc,0.1 mg.kg-1)group,model group and normal control group.Rats were injected carbon tetrachloride(CCl4) subcutaneously two times a week to induce hepatofibrosis as well as ig administration of TF or colchicines.After 10 weeks,the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(AKP),albumin(ALB),globulin(GLB) in serum and hydroxyproline(Hyp),malondialdehyde(MDA),glutathione peroxidase(GSH-Px) in liver tissue were determined,and liver histological changes were investigated by hematoxylin-eosin(HE) staining.Result: Compared with model group,TF significantly reduced the levels of ALT,AST,AKP and GLB in serum(P < 0.01),and decreased Hyp,MDA level in liver tissue(P < 0.05) while ALB,A/G,GSH-Px were elevated significantly(P < 0.01).Pathological examination revealed that TF had therapeutical effect on experimental hepatofibrosis.Conclusion:TF has better effects on protecting hepatocyte,relieving liver injury and against hepatofibrosis.更多还原