【摘要】 目的:观察CD4+T细胞及其共刺激分子(CSF)在湿热型溃疡性结肠炎(UC)大鼠肠黏膜中的表达及分布,探讨黄芩汤治疗该病可能的免疫学机制。方法:采用高脂、高糖饲料联合人工气候箱法造出湿热型体质,结合三硝基苯磺酸(TNBS)灌肠法建立湿热型UC模型;造模成功后将大鼠分为模型组、黄芩汤组及美沙拉嗪组,用免疫组化SP法检测大鼠结肠黏膜CD4+T细胞,CD28,CD152,OX40T细胞亚型的表达及分布,通过图像数码采集系统及Image pro plus 6.0软件分析平均光密度,以检测各分子表达含量。结果:与正常对照组比较,造模大鼠结肠中CD4+T细胞、CD28,CD152,OX40等T细胞亚型升高(P<0.05),主要分布于黏膜下层及固有肌层;治疗后,黄芩汤和美沙拉嗪组大鼠结肠中CD4+T细胞,CD28,OX40T细胞亚型表达均下降(P<0.05),但CD152的表达无明显变化且维持在模型组水平;黄芩汤组及美沙拉嗪组以上各分子的表达均无统计学差异。结论:黄芩汤可能通过调节CD4+T细胞的CSF的表达含量从而发挥对溃疡性结肠炎的免疫调节作用。更多还原

【Abstract】 Objective:To observe the expression and localization of CD4+T cell and its co-stimulator factors(CSF) in the mucosa of ulcerative colitis(UC)rat with damp-and-heat syndrome,and to explore the conceivable immunology mechanism of Huangqin decoction to treat the disease.Method:The model of UC rats with damp-andheat syndrome were established by giving the high sugar fat food and put into an artificial bioclimatic chamber,resulting in damp-and-heat body constitution and then clysmaed with 2,4,6-trinitro-benzene-sulfonic acid.After that the rats were divided into the model group,Huangqin decoction group and mesalazine group.SP of Immunohistochemistry was used to determine the expression and localization of CD4+T cell,CD28,CD152,OX40 T cell subsets in mucosa.The above antigens were tested using Image pro plus 6.0,an image acquisition system based on the digital camera and software,by analyzing their mean density.Result:Compared with the normal rats(n=15),the expression of CD4 + T cell,CD28,CD152 and OX40 T cell subsets in mucosa increased for the model rats(n=38 in total and P<0.05),and targeted to distribution in the submucosa and inherent layers.Compared with the model control group(n=12),CD4+T cell,CD28 and OX40 in both Huangqin decoction(n=13) and mesalazine group(n=12) decreased(P<0.05),but CD152 did not,kept in the level of model gruop.There was no statistical difference of the level of the above antigens between Huangqin decoction(n=13) and mesalazine group(n=12,P>0.05).Conclusion: It is possible that the expression of the CSF for CD4+T cells maybe the immunology mechanism of the decotion to treat UC.更多还原