【摘要】 目的:建立红花药材的HPLC标准指纹图谱,为科学评价和有效控制其质量提供可靠的方法。方法:用HPLC方法测定了46批市售红花药材,采用中药色谱指纹图谱相似度评价系统,建立红花的标准指纹图谱并计算了每批药材的相似度。结果:46批市售红花药材的HPLC指纹图谱主要有12个共有峰,其中10个共有峰得到确认,相似度在0.8以上占76%。结论:红花药材可通过与标准指纹图谱比较评价质量,本方法可用于红花药材质量评价。更多还原

【Abstract】 Objective:To establish a sensitive and specific HPLC method and a standard fingerprint for quality control of Carthamus tinctorius.Method: About 46 samples from different sources were analyzed by HPLC.By the similarity evaluation system for chromatographic fingerprint of traditional chinese medicine(Version 2004A),mean chromatogram was generated as the representative standard fingerprint and the similarity of each chromatogram against the standard chromatogram was also calculated.Result: Twelve main marker peaks were selected in the standard fingerprint and 10 peaks of them were identified.The similarity of 76% samples was over 0.8.Conclusion: The quality control of crude drug can be evaluated by comparing with the standard fingerprint.The method can be applied for quality assessment of C.tinctorius.更多还原