【摘要】 在6种不同盐度(34、32、30、28、26和22)激活液、3种不同K+浓度(25 mmol/L、30 mmol/L和35 mmol/L)稀释液和不同保存时间(0 h、24 h、48 h、72 h和96 h)条件下,对日本鳗鲡(Anguilla japonica)精子的活力进行观察和测定。结果表明,激活液盐度为30时精子活率和运动精子百分比均最高,对应平均曲线运动速度(VCL)、平均直线运动速度(VSL)和平均路径速度均最高,由此推测自然界中日本鳗鲡产卵环境的盐度在30左右;稀释液中K+浓度为30 mmol/L时精子活率和运动精子百分比均最高,对应平均曲线运动速度(VCL)、平均直线运动速度(VSL)和平均路径速度(VAP)均显著高于25 mmol/L和30 mmol/L(P<0.05),据此推算出精子稀释液的最佳渗透压为796.68 kPa;随低温保存时间的延长,精子活率逐渐降低,稀释液中K+浓度为30 mmol/L时保存时间可达到96 h,并且精子活率与K+浓度为25 mmol/L、35 mmol/L时相比较,显著升高(P<0.05)。更多还原

【Abstract】 Sperm motility of Anguilla japonica treated with six activation mediums with different salinities(34,32,30,28,26 and 22),three extender solution with different potassium ion concentration(25 mmol/L,30 mmol/L,35 mmol/L) and different conservation time(0 h,24 h,48 h,72 h and 96 h) was observed and measured.The results showed that: when activation medium salinity was 30,both sperm motility and percentage of motile sperms were the highest,meanwhile the curvilinear velocity(VCL),the straight line velocity(VSL) and the angular path veloc-ity(VAP) were the highest.It could be concluded that the spawning salinity of Anguilla japonica may be 30 in natural environment.When potassium ion concentration in extender solution was 30 mmol/L,both sperm motility and percentage of motile sperms were the highest,meanwhile the curvilinear velocity(VCL),the straight line ve-locity(VSL) and the angular path velocity(VAP) were markedly higher than those under other concentration lev-els(P<0.05).So the best osmotic pressure of extender solution may be 796.68 kPa.As the conservation time ex-tended,sperm motility reduced gradually.When potassium ion concentration in extender solution was 30 mmol/L,conservation time of sperm could reach 96 h and sperm survival rate was higher than in 25 mmol/L and 35 mmol/L potassium ion.更多还原