【摘要】 目的筛选白纹伊蚊基因表达定量PCR研究中适合的内参基因。方法采用实时荧光定量PCR技术,对β-ac-tin、BTF3a、rsp5、rsp27a、superoxide、rspL40六个看家基因的mRNA表达水平进行了探讨。结果除rsp27a基因扩增效率高于设定值被剃除外,余5个基因在不同组织中的表达稳定度为rspL40,BTF3a>rsp5>β-actin>superoxide;吸血不同时相表达稳定度为rspL40,rsp5>superoxide>BTF3a>β-actin。结论 rspL40,BTF3在不同组织中表达最稳定;rspL40,rsp5在吸血不同时相表达最稳定。更多还原

【Abstract】 To select the internal control genes for real-time quantitative PCR(qPCR) in gene expression analysis in Ae.albopictus,six housekeeping genes were evaluated in this study.According to the analysis by the geNorm programme,the expression stability of the five genes in different tissues and different blood-feeding phases varied and the orders were rspL40,BTF3a>rsp5> β-actin,superoxide and rspL40,rsp5>superoxide>BTF3a>β-actin respectively with exception of the rsp27a which amplification efficiency was higher than the setting value.These results imply that rspL40,BTF3a has the most stable expression in different tissues,whereas rspL40,rsp5 stably were expressed most stably in different blood feeding phases.更多还原