【摘要】 目的:探讨甲基鸟嘌呤DNA甲基转移酶(Methylguanine-dna methyltransferase,MGMT)在脑胶质瘤细胞和胶质瘤干细胞(Brain glioma stem cells,BGSC)中的表达情况。方法:由手术切除的多形性胶质母细胞瘤中分离培养胶质瘤细胞和BGSC;应用免疫细胞化学染色和流式细胞术(Flow cytometry,FCW)鉴定BGSC;应用RT-PCR法测定MGMT mRNA在胶质瘤细胞、BGSC和正常脑组织中的表达。结果:本研究中获得的BGSC具有典型的BGSCs细胞生物学特征和免疫学标记;RT-PCR检测结果表明MGMT mRNA在BGSC中的相对表达量明显高于胶质瘤细胞(0.562±0.021 vs 0.112±0.023,P<0.01),而胶质瘤细胞MGMTmRNA的相对表达量明显高于正常脑组织(0.112±0.023 vs 0.005±0.001,P<0.01)。结论:多药耐药基因MGMT主要在BGSC而非胶质瘤细胞中表达,其可能是BGSC产生多药耐药性的主要分子机制之一。更多还原

【Abstract】 Objective:To explore the expression of MGMT gene in glioma stem cells and glioma cells derived from glioblastoma.Methods:BGSC was isolated from glioblastoma and cultured in vitro,and was identified by immunocytochemical stain and flow cytometry.Relative expression of MGMT gene in BGSC and in glioma cells by RT-PCR was analyzed.Results:BGSC from glioblastoma could express Nestin and CD133 antigen,self-renew and differentiate into astrocyte and neuron in vitro.RT-PCR results demonstrated the relative expression of MGMT gene in BGSC was markedly higher than in glioma cells(0.562±0.021 vs 0.112±0.023,P<0.01),and the relative expression of MGMT gene in glioma cells was markedly higher than in normal brain tissue(0.112±0.023 vs 0.005±0.001,P<0.01).Conclusion:The expression of MGMT gene in BGSC is markedly higher than in glioma cells.MGMT may be the key molecular biology mechanism that leads to drug resistance in BGSC.更多还原