【摘要】 目的:调查17个Y染色体短串联重复序列(Y-Short tandem repeat,Y-STR)基因座及其单倍型在新疆巴州维吾尔族人群中的分布情况。方法:应用AmpFlSTR YfilerTM荧光标记复合扩增系统,对149名维吾尔族无关男性个体血样进行17个Y-STR位点的复合扩增,用ABI 3130XL遗传分析仪对扩增产物进行检测分析。结果:DYS456、DYS389Ⅰ/Ⅱ、DYS390、DYS458、DYS19、DYS385a/b、DYS393、DYS391、DYS439、DYS635、DYS392、Y-GATA-H4、DYS437、DYS438、DYS448各位点遗传多样性(Genetic diversity,GD)值分布在0.498 2～0.948 5之间;17个Y-STR位点共观察到单倍型148种,其单倍型多样性(Haplo-type diversity,HD)值为0.999 86。结论:新疆巴州维吾尔族17个Y-STR位点具有丰富的遗传多样性,可为父权鉴定和父系进化研究提供有价值的遗传学资料。更多还原

【Abstract】 Objective:To investigate the allelic and haplotype frequency distribution of seventeen short tandem repeat loci of Y chromosome in Xinjiang Uygur population in Bazhou.Methods:The template DNAs were extracted from blood samples of 149 unrelated Uygur male individuals,and seventeen Y-STR loci were amplified by the AmpFlSTR YfilerTM.The PCR products were analyzed and genotyped with ABI3130XL Sequencer.Results:The values of genetic diversity of DYS456,DYS389Ⅰ/Ⅱ,DYS390,DYS458,DYS19,DYS385a/b,DYS393,DYS391,DYS439,DYS635,DYS392,Y-GATA-H4,DYS437,DYS438,and DYS448 were between 0.4 982 and 0.9 485.A total of 148 different haplotypes were observed,and the value of genetic diversity of haplotypes was 0.99 986.Conclusion:The 17 Y-STR loci in Xinjiang Uygur population in Bazhou are highly polymorphic and suitable for paternity testing and paternal genetic lineage evolution.更多还原