【摘要】 目的:探讨老年大鼠胼胝体额区有髓神经纤维、髓鞘的结构变化和髓鞘碱性蛋白(Myelin basic protein,MBP)的表达变化,以及二者间的关系。方法:运用透射电子显微镜和新的体视学方法分别对年轻组和老年组雌性Long-Evans大鼠胼胝体额区有髓神经纤维和髓鞘进行定量研究,运用Western blot对年轻组和老年组大鼠胼胝体额区MBP表达进行定量研究。结果:与年轻组相比,老年组胼胝体额区体积存在显著性降低,有髓神经纤维总体积显著性下降,21.5 kD MBP表达量显著性降低。21.5 kD MBP的表达降低与有髓神经纤维总体积、髓鞘总体积的降低之间呈正相关。结论:胼胝体存在显著性老年萎缩,胼胝体额区有髓神经纤维和MBP的老年改变可能与老年大脑额叶功能下降有关。更多还原

【Abstract】 Objective:To explore the age-related changes of the myelinated fibers,myelin sheath and the expression of myelin basic protein in the frontal region of the corpus callosum in old rats,and their relationship.Methods:The myelinated fibers and sheathes in the frontal region of corpus callosum of young and old-aged female Long-Evans rats were quantitatively studied using electron microscope and unbiased stereological techniques.Myelin basic proteins in the frontal region of corpus callosum of young and old-aged female rats were quantitatively studied using Western blot.Results:When compared with young female rats,the total volume of the frontal region of the corpus callosum and the total volume of the myelinated fibers in the frontal region of old corpus callosum were significantly degraded.The expression of 21.5 kD MBP was significantly decreased in old rats.The reduction of the expression of 21.5 kD MBP,the reduction of the total volume of the myelinated fibers and the total volume of the myelin sheathes were positively correlated.Conclusion:There is age-related atrophy of the frontal region of corpus callosum.The age-related changes of the myelinated fibers and MBP in the frontal region of corpus callosum may be related to the decline of frontal lobe functions with aging.更多还原