【摘要】 目的建立高效液相色谱-电喷雾串联质谱法测定人血浆中厄洛替尼的浓度。方法 血浆样品经甲醇蛋白沉淀后,以甲醇-(0.1%甲酸+5mmol.L-1 NH4Ac)(60∶40,v/v)为流动相,采用Ultimate XB-C18(4.6mm×150mm,5μm)色谱柱进行分离,流速为0.9mL·min-1,通过电喷雾离子化串联质谱,以多反应监测(MRM)方式进行检测。结果厄洛替尼的线性范围为1～2 000ng·mL-1,平均方法回收率在103.5%～109.0%,日内和日间变异均<15%。结论本法简单、快速、灵敏、重现性好,成功应用于厄洛替尼的血药浓度监测。更多还原

【Abstract】 Objective To develop a liquid chromatography-tandem mass spectrometry to determine erlotinib in human plasma. Methods After protein precipitation with methanol,erlotinib and internal standard were chromatographically separated on an Ultimate XB C18 column(4.6 mm×150 mm,5 μm) with the mobile phase containing(0.1% formate and 5 mmol·L-1 ammonium acetate buffer) and acetonitrile(40∶60,v/v) at 0.9 mL·min-1.The electronic spray ion tandem mass spectrum with the positive mode and multitude reaction monitor(MRM) were used to detect the analytes. Results The linear range of the calibration curves was 1-2 000 ng·mL-1 for erlotinib.The intra-and inter-day precision was less than 15%.The methodology recovery ranged from 103.5% to 109.0%. Conclusion The present method is sensitive,effective and reliable,which has been successfully used in the pharmacokinetic studies of erlotinib after oral administration in healthy Chinese volunteers.更多还原