【摘要】 目的对逍遥方新制剂的HPLC指纹图谱进行研究,以更好的控制其质量。方法采用HPLC法,以乙醇-0.1%磷酸为流动相二元梯度洗脱,流速1.0 mL.min-1,柱温35℃,检测波长230 nm,进样量:10μL,检测时间70 min。结果通过方法学考察建立了逍遥方新制剂的指纹图谱检测方法,并对10批逍遥方新制剂进行指纹图谱检测分析共检出共有峰17个,10批样品的相似度均>0.9。结论该法具有可操作性,能有效地控制逍遥方新制剂的质量。更多还原

【Abstract】 Objective To determine the compound HPLC fingerprint of new Xiaoyao preparation,and to control its quality.Methods HPLC was adopted with methyl acetonitrile-water(containing 0.1%phosphoric acid),the flow rate was 1.0 mL·min-1,the column temperature was 35 ℃,the detection wavelength was 230 nm,and the detection time was 70 min.We established a test method for new Xiaoyao preparation by methodology inspection,inspected and analyzed 10 batches of new Xiaoyao preparation.Results Fingerprint of new Xiaoyao preparation was established,10 batches of samples showed in the similarity of more than 0.9,and 17 joint chromatographic peaks detected.Conclusion The method is reliable and helpful in the effective control of new Xiaoyao preperation.更多还原