【摘要】 【目的】研究干旱胁迫对小麦代换系旗叶叶绿素荧光诱导动力学的影响以及外源染色体对叶绿素荧光参数的调控效应。【方法】以中国春(Chinese Spring,CS)-人工合成六倍体小麦(Synthetic hexaploid wheat,Synthetic 6x)代换系及其亲本为材料,于2005—2007年测定不同水分处理条件下旗叶叶绿素荧光诱导的动力学参数初始荧光(Fo)、最大荧光(Fm)、PSⅡ的原初光能转化效率(Fv/Fm)和PSⅡ潜在活性(Fv/Fo)在各生育时期的变化。【结果】在正常水分条件下,5B代换系在孕穗期、开花期以及灌浆期的Fo和Fm均显著或极显著高于中国春。在干旱胁迫条件下,PSⅡ的光化学活性受到抑制,不同代换系与亲本的Fo增加,而Fm、Fv/Fm、Fv/Fo降低;3A代换系的Fo始终显著或极显著低于中国春,4D代换系的Fm始终显著或极显著高于中国春,3A和7A代换系Fv/Fm和Fv/Fo始终显著或极显著高于中国春。【结论】在正常水分条件下Synthetic 6x的5B染色体上可能存在高Fo和Fm基因;干旱胁迫条件下,Synthetic 6x的3A染色体上可能存在调控Fo增高的有利基因,4D染色体上可能存在调控Fm增高的有利基因,3A和7A染色体上可能存在调控Fv/Fm以及Fv/Fo增高的有利基因。更多还原

【Abstract】 【Objective】 Chromosome substitution lines between Chinese Spring and synthetic hexaploid wheat(CS-Synthetic 6x) were used to study the regulation effect of foreign added chromosome on chlorophyll fluorescence parameters under drought stress.【Method】 The chlorophyll fluorescence parameters,including Fo,Fm,Fv/Fm and Fv/Fo in flag leaves at different developing stages in different water treatments were measured during 2005-2007.【Result】 The results showed that Fm and Fo of 5B substitution line were significantly different from Chinese Spring in the control treatment.Drought stress resulted in inhibition of PSⅡ photochemical activity and reduction of Fm,Fv/Fm,Fv/Fo as well as accumulation of Fo;Fo of 3A substitution line and Fm of 4D substitution line were significantly different from Chinese Spring;Fv/Fm and Fv/Fo of 3A,7A substitution lines were significantly different from Chinese Spring from booting stage to filling stage under drought stress.【Conclusion】 The genes regulating Fo and Fm may be located on 5B chromosome of Synthetic 6x in the control treatment.In drought treatment the genes regulating Fo and Fm may be located on 3A and 4D chromosome,respectively,and the genes regulating Fv/Fm and Fv/Fo may be located on 3A and 7A chromosomes,the problem is up for further study.更多还原