【摘要】 统计了辽宁省3个引进猪种:长白、大白和杜洛克的产仔数记录,并采用PCR-RFLP方法检测3个猪种ESR、FSH-β和PRLR基因的多态性,分析3个基因的单基因及合并基因型对产仔数的影响。结果表明,ESR、FSH-β和PRLR基因都存在AA、AB和BB3种基因型,在各猪群内基因型分布差异不显著(P>0.05),各基因均处于遗传平衡状态。单基因分析表明,ESR和FSH-β基因型产仔数的最小二乘均值具有按AA、AB、BB递增的趋势,BB型为优良基因型,而PRLR基因的优良基因型为AA型。ESR、FSH-β和PRLR基因的合并分析表明BBBBAA为最优合并基因型,合并后效应显著(P<0.05)或极显著(P<0.01),且合并基因型的遗传效应高于单基因型效应。因此,可以利用合并基因型对猪的产仔数进行标记辅助选择。更多还原

【Abstract】 To statistical analysis of the litter size record for three introduced swine (Landrace,Large Yorkshire,Duroc) of Liaoning province,single genotype effects as well as combined genotype effects were all studied,PCR-RFLP were applied to detect polymorphism of three genes.The results showed that there were there genotypes:AA,AB and BB for ESR,FSH-β and PRLR gene,the distributed difference of genotype was not significant (P > 0.05),it was in genetic equilibrium for three genes.The results of single genotype effects showed that all LSM of the litter size had a gradually increasing tendency AA,AB and BB in turn,BB genotype of ESR and FSH-β gene were the superior one,but it was AA for PRLR gene.The results of combined genotype effects showed that BBBBAA was the dominant combination,there were significant differences (P < 0.05) or very significant differences (P < 0.01) in the combined genotypes effects,and the combined genotypes effects was higher than single genotypes effects.In conclusion,reproductive traits of swine could be improved by applying marker-assisted selection of combining genotypes.更多还原