【摘要】 实验比较了烟蚜SSR-PCR产物聚丙烯酰胺凝胶电泳2种改良银染方法,目的是为初用者在选择银染方法时提供参考。用非变性聚丙烯酰胺凝胶电泳检测烟蚜SSR-PCR的产物,结果表明银染方法2灵敏度高、简便省时,更适于对烟蚜遗传多样性的研究。更多还原

【Abstract】 Two ways of DNA improved silver staining methods for polyacrylamide gels electrophoresis on Myzus persicae(Sulzer) were compared in this article for first detection.The detected results with the technique of SSR markers by non-denaturant polyacrylmaaide gel eletrophoresis indicated that the second method was characterized as more sensitive and simple steps when compared with the first method.And for saving time and work the second method was more appropriate to the genetic diversity of Myzus persicae(Sulzer).更多还原