【摘要】 通过对小麦面粉膨胀势进行初步定位,可以为小麦品质遗传改良和相关基因的精细定位和克隆提供理论依据。以小麦‘花培3号’和‘漯麦4号’的双单倍体群体为材料,通过一年两点试验,研究了面粉膨胀势的遗传方式,并采用复合区间作图法对其进行QTL分析。结果发现,面粉膨胀势由微效多基因控制,为数量性状。QTL定位结果表明,在北京点定位到2个与面粉膨胀势相关QTLs,位于4A染色体上。这两个位点均来自母本‘花培3号’等位基因的增效作用,其效应值分别为0.4298和0.5948。河南点只检测到1个与面粉膨胀势相关QTL,也位于4A染色体上。该位点来自母本‘花培3号’等位基因的减效作用,其效应值为0.3024。3个QTLs位于4A染色体上的不同标记区间,共解释面粉膨胀势37.92%的表型变异。两地没有检测到共同的QTL。尽管北京点的两个位点贡献率分别为11.29%和21.48%,但不是稳定的QTL,可能在小麦面粉膨胀势分子标记辅助选择上应用不是很大。更多还原

【Abstract】 To offer theoretical reference for wheat quality genetic improvement research and fine mapping of related genes,the Double Haploid Lines(DH)derived from the cross between’Huapei 3’and’Luomai 4’ were used to analyze the genetic way and to identify QTLs for flour swelling power.Composite Interval Mapping was used in QTL Mapping.The results were described as below:swelling character was quantitative in nature,and controlled by many genes with small effects.Two QTLs for flour swelling were identified and mapped on chromosome 4A by analyzing the DH population in Beijing.They were from the additive effect of’Huapei3’ alleles,their effect values were 0.4298 and 0.5948,respectively.However,one QTL was identified and mapped on chromosome 4A by analyzing the DH population in Henan Province.The location was from the reductive effect of’Huapei3’alleles,the effect value was 0.3024.Three QTLs were located in different intervals on chromosome 4A,explaining 37.92% of the phenotypic variations.No common QTLs was detected in two areas.Although the contribution rate of two QTLs were 11.29% and 21.48% in Beijing and were not stable QTLs.Maybe their applications were not of great value in Marker-assisted Selection for flour swelling power.更多还原