【摘要】 以低温导入法将钙离子荧光探针Fluo-3/AM导入拟南芥叶片细胞中,利用LAS AF(LeicaApplication Suite-Advanced Fluorescence)软件记录肝素对茉莉酸(JA)诱导的胞内钙离子荧光强度的变化。结果显示,经不同浓度的肝素预处理后,拟南芥叶细胞中胞内钙离子的荧光强度降低,再用100μmol/L JA处理时,其荧光强度升高,但仅与未经肝素处理的荧光强度相当。实验证明,肝素预处理可抑制JA诱导的胞内钙离子浓度的升高。更多还原

【Abstract】 Arabidopsis thaliana leaves were labeled by fluorescent probe Fluo-3/AM under low temperature at 4℃ to measure the fluorescent intensity of intracellular Ca 2 + which was pretreated with heparin on jasmonic acid (JA)-induced. The results showed that the fluorescent intensity of [Ca 2 + ] cyt was reduced after pretreated with different concentration of heparin, and then treated with 100 μmol/L JA, the fluorescent intensity of [Ca 2 + ] cyt was close to the fluorescent intensity which was not pretreated with heparin. The experiment showed that the pretreatment with heparin could inhibit the increase of the intracellular Ca 2+ concentration significantly which JA-induced in leaves of Arabidopsis thaliana.更多还原