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IP3敏感的钙离子通透性通道参与茉莉酸诱导的钙动员

IP3 Sensitive Calcium Channel Involved in the Jasmonic Acid Induced Calcium Mobilization

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【作者】 李洋洋杜希华于涌鲲赵福宽孙清鹏

【Author】 Li Yangyang 1,2 , Du Xihua 1 , Yu Yongkun 2 , Zhao Fukuan 2 , Sun Qingpeng 2 ( 1 College of Life Sciences, Shandong Normal University, Jinan 250014; 2 College of Biotechnology, Beijing University of Agriculture, Beijing 102206)

【机构】 山东师范大学生命科学学院北京农学院生物技术学院

【摘要】 以低温导入法将钙离子荧光探针Fluo-3/AM导入拟南芥叶片细胞中,利用LAS AF(LeicaApplication Suite-Advanced Fluorescence)软件记录肝素对茉莉酸(JA)诱导的胞内钙离子荧光强度的变化。结果显示,经不同浓度的肝素预处理后,拟南芥叶细胞中胞内钙离子的荧光强度降低,再用100μmol/L JA处理时,其荧光强度升高,但仅与未经肝素处理的荧光强度相当。实验证明,肝素预处理可抑制JA诱导的胞内钙离子浓度的升高。

【Abstract】 Arabidopsis thaliana leaves were labeled by fluorescent probe Fluo-3/AM under low temperature at 4℃ to measure the fluorescent intensity of intracellular Ca 2 + which was pretreated with heparin on jasmonic acid (JA)-induced. The results showed that the fluorescent intensity of [Ca 2 + ] cyt was reduced after pretreated with different concentration of heparin, and then treated with 100 μmol/L JA, the fluorescent intensity of [Ca 2 + ] cyt was close to the fluorescent intensity which was not pretreated with heparin. The experiment showed that the pretreatment with heparin could inhibit the increase of the intracellular Ca 2+ concentration significantly which JA-induced in leaves of Arabidopsis thaliana.

【关键词】 拟南芥钙离子肝素茉莉酸
【Key words】 Arabidopsis thalianaCa 2+heparinjasmonic acid
【基金】 国家自然科学基金“环核苷酸门控的离子通道在拟南芥茉莉酸信号通路中的作用”、“AtCNGC2/AtCNGC19在茉莉酸诱导的钙离子动员中的分子机制”(30700428,30911130166);北京市自然科学基金“AtCNGC离子通道在茉莉酸诱导的钙动员中的作用”(5072009);北京市科技新星计划“AtCNGC2或AtCNGC4在茉莉酸诱导的钙离子动员中的作用”(2006B26)
  • 【文献出处】 中国农学通报 ,Chinese Agricultural Science Bulletin , 编辑部邮箱 ,2011年01期
  • 【分类号】Q946
  • 【被引频次】4
  • 【下载频次】177
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